Educations

• P.H.D, 2012, straight Ph.D of Biotechnology, university of Tehran
• M.S, ---, Straight Ph. D of Biotechnology, university of tehran
• B.S, ---, Straight Ph. D of Biotechnology, university of tehran

National Articles

• Asad, Sedigheh, Seyed Mohammad Mehdi Dastgheib, and Mohammad Amozegar. "Optimization for decolorization of azo dye Remazol Black by a Halomonas strain using the Taguchi approach." Progress in Biological Sciences 4, no. 1 (2014): 33-42.
• Master Thesis
• Mahdavi Zafarghandi, Farzaneh, Mohammad Amozegar, Sedigheh Asad, and maryam Siroosi. "Evaluation of biological decolorization of the textile azo-dyes by halophilic and halotolerant actinomycetes." Color Science and Technology 11, no. 2 (2017): 145-152.
• Selseleh Hassan-kiadehi, Masoomeh, Mohammad Amozegar, and Sedigheh Asad. "Evaluation of biodecolarization of the textile azo dye by halophilic archaea." Biological Journal of Microorganism 6, no. 23 (2017): 1-17.
• fahimeh hajipour, Sedigheh Asad, Mohammad Amozegar, and Khosro Khajeh. "Synthesis of the MPA- Capped CdSe/ZnS Quantum Dots and Study the Quenching Effect of Azo Dyes on Them." Modares Journal of Biotechnology 10, no. 3 (2019): 407.
• parisa norouzi fard, samar mortazavi, Sedigheh Asad, and nasrin hasan zadeh. "HEAVY metal content of sediments; MARINE sediments; HEAVY metals; ECOSYSTEM health; PERCHLORIC acid; NITRIC acid." Iranian Journal of Health & Environment 3, no. 11 (2018): .
• parisa norouzi fard, samar mortazavi, Sedigheh Asad, and nasrin hasan zadeh. "Toxic Elements Bioavailability from Total Amount in Persian Gulf Surface Sediments." Tarbiyat Modares 8, no. 1 (2019): 359-369.
• parisa norouzi fard, samar mortazavi, Sedigheh Asad, and . "Using Saccostrea cucullata as a biomonitor of heavy metals (Cu, Pb, Zn, Cd, Ni, and Cr) in water and sediments of Qeshm Island, Persian Gulf." ECOPERSIA 8, no. 3 (2020): 181-190.
• ph.D Thesis

International Articles

• Asad, Sedigheh Asad, M A Amoozegar , A A Pourbabaee , Mohammad Nabi Sar Bouloki, and S M M Dastgheib . "Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria." Bioresource Technology 98, no. 11 (2007): 2082-2088.
• Asad, Sedigheh Asad, Khosro Khajeh , and Naser Ghaemi. "Investigating the Structural and Functional Effects of Mutating Asn Glycosylation Sites of Horseradish Peroxidase to Asp." APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY PART A ENZYME ENGINEERING AND BIOTECHNOLOGY 164, no. 4 (2011): 454-463.
• Amozegar, Mohammad, Mahbod Hajighasemi , Javad Hamedi, Sedigheh Asad Asad, and Antonio Ventosa . "Azo dye decolorization by halophilic and halotolerant microorganisms." Annals of Microbiology 61, no. 2 (2011): 217-230.
• Asad, Sedigheh, S F Torabi , M Fathi Roudsari , Naser Ghaemi, and K Khajeh . "Phosphate buffer effects on thermal stability and H2O2 - resistance of horseradish peroxidase." INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 48, no. 4 (2011): 566-570.
• Eslami, maryam, Mohammad Amozegar, and Sedigheh Asad Asad. "Isolation, cloning and characterization of an azoreductase from the halophilic bacterium Halomonas elongata." International Journal of Biological Macromolecules 85, no. 4 (2015): 111-116.
• Asad, Sedigheh, Bahareh Dabirmanesh, and . "Phenol removal from refinery wastewater by mutant recombinant horseradish peroxidase." BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 61, no. 2 (2014): 226-229.
• Asad, Sedigheh, Bahareh Dabirmanesh , Nasser Ghaemi, Seyed Masoud Etezad , and Khosro Khajeh . "Studies on the Refolding Process of Recombinant Horseradish Peroxidase." MOLECULAR BIOTECHNOLOGY -, no. --- (2012): -.
• Asad, Sedigheh, seyed mohammad mehdi Dastgheib, and khosro khajeh. "Construction of a horseradish peroxidase resistant towards hydrogen peroxide by saturation mutagenesis." BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 63, no. 6 (2015): 1-6.
• ghasemi, ali, Sedigheh Asad, Mahboubeh Kabiri Renani00, and Bahareh Dabirmanesh. "Cloning and characterization of Halomonas elongata L-asparaginase, a promising chemotherapeutic agent." Applied Microbiology and Biotechnology 101, no. 19 (2017): 7227-7238.
• shirazian, pejman, Sedigheh Asad Asad, and Mohammad Amozegar. "THE POTENTIAL OF HALOPHILIC AND HALOTOLERANT BACTERIA FOR THE PRODUCTION OF ANTINEOPLASTIC ENZYMES: L-ASPARAGINASE AND L-GLUTAMINASE." EXCLI Journal 15, no. 0 (2016): 268-279.
• Babaei, Parizad, Sayed-Amir Marashi, and Sedigheh Asad Asad. "Genome-scale reconstruction of the metabolic network in Pseudomonas stutzeri A1501." Molecular BioSystems 11, no. 11 (2015): 3022-3032.
• alishah, khadijeh, Sedigheh Asad Asad, khosro Khajeh, and neda Akbari. "Utilizing Intein-mediated Protein Cleaving for Purification of Uricase, A Multimeric Enzyme." Enzyme and Microbial Technology 1, no. 1 (2016): 1-21.
• Nakhaee, Narjes, Sedigheh Asad, khosro Khajeh, , and Mohammad Amozegar. "Improving the thermal stability of azoreductase from Halomonas elongata by introducing a disulfide bond via site-directed mutagenesis." BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 1, no. 1 (2018): 1.
• Mandana azari, Sedigheh Asad, and Mohammad Reza Mehrnia. "Heterologous production of porcine derived antimicrobial peptide PR-39 in Escherichia coli using SUMO and intein fusion systems." PROTEIN EXPRESSION AND PURIFICATION 169, no. 1 (2020): 105568.
• selseleh, masoomeh, Mohammad Amozegar, Sedigheh Asad, and maryam siroosi. "Exploring the potential of halophilic archaea for the decolorization of azo dyes." Water Science and Technology 77, no. 6 (2018): 1602-1611.
• S.m.m Dastgheib, Mohammad Amozegar, Elahe Allahi, Sedigheh Asad, and Ibrahim Mohamed Banat . "Bioemulsifier production by a halothermophilic Bacillus strain with potential applications in microbially enhanced oil recovery." BIOTECHNOLOGY LETTERS 30, no. 2 (2008): 263-270.
• Motahareh Sobat, Sedigheh Asad, Mahbobe Kabiri, and . "Metagenomic discovery and functional validation of L-asparaginases with anti-leukemic effect from the Caspian Sea." IScience 24, no. 24 (2021): 101973.
• Reyhaneh khaleghi, and Sedigheh Asad. "Heterologous expression of recombinant urate oxidase using the intein‑mediated protein purification in Pichia pastoris." 3 Biotech 11, no. 1 (2021): .
• Atefeh Khodakarami, Bahareh Dabirmanesh, Sedigheh Asad, and mohammad khaledi. "Enhanced Solubility and One-Step Purification of Functional Dimeric Carboxypeptidase G2." BIOCHEMISTRY-MOSCOW 86, no. 2 (2021): 190-196.

National Conferences

• Asad, Sedigheh, Mohammad Amozegar, Mohammad Nabi Sar Bouloki, and Ahmad Ali Pourbabaee Pourbabaei. "Application of halophilic and halotolerant bacteria in clean up of textile dye mill effluents." 4th Conference of Biothecnology in Iran, Kerman, August 15-17, 2005 .
• Asad, Sedigheh, , and Nasser Ghaemi. "Studies on the effects of site directed mutagenesis on the properties of Horseradish peroxidase." 7th national biotechnology congress of I.R. Iran, Tehran, September 12-14, 2011 .

International Conferences

• shirazian, Pejman, Sedigheh Asad Asad, and Mohammad Amozegar. "Screening of L-glutaminase producing halophilic bacteria and media optimization by response surface methodology." 15th international and iranian congress of microbiology, Tehran, August 26-28, 2014 .
• eslamy, maryam, Mohammad Amozegar, and Sedigheh Asad Asad. "molecular cloning and characterization of the gene coding for azoreductase from Halomonas elongata DSM 2581." 15th international iranian conference of microbiology, Tehran, August 26-28, 2014 .
• Asad, Sedigheh Asad, Pejman shirazian, and Mohammad Amozegar. "studies on the potential of moderately halophilic bacteria for the production of drug enzymes." First international and 13th iranian genetics congress, Tehran, May 24-26, 2014 .
• Asad, Sedigheh Asad, Pejman shirazian, and Mohammad Amozegar. "Screening and isolation of halophilic bacteria producing extracellular l-asparaginase from Urmia salt lake." first international and 13th iranian genetics congress, Tehran, May 24-26, 2014 .
• Nakhaee, Narjes, Sedigheh Asad, khosro Khajeh, , and Mohammad Amozegar. "Studies on the effects of point mutations on the stability of azoreductase from Halomonas elongata." 2nd international and 10th national biotechnology congress of Islamic republic of Iran, Tehran, August 29-31, 2017 .
• Amozegar, Mohammad, , Elahe Allahi, Sedigheh Asad, and . "Identification of halotolerant bioemulsifier producing bacteria from persian oil fields." FEMS congress of europian microbiologist, Madrid, July 4-8, 2006 .
• Amozegar, Mohammad, Sedigheh Asad, Mohammad Nabi Sar Bouloki, Ahmad Ali Pourbabaee Pourbabaei, and . "Identification of halophilic azo dye degrading bacteria from textile effluents in salty soils of Iran." FEMS congress of Europian Microbiologist, Madrid, July 4-8, 2006 .
• khodakarami, atefeh, Bahareh Dabirmanesh , Sedigheh Asad, and Khosro Khajeh. "Purification of a multimeric enzyme carboxypeptidase G2 by intein -mediated protein splicing." international congress on Medicine, Public health and biological sciences(MPHBS), Tehran, September 18-19, 2016 .
• Amozegar, Mohammad, , , and Sedigheh Asad. "Isolating biosurfactant producing microflora of petroleum reservoirs capable of tolerating high salinity and temperature." Extremophiles 2006,, September 17-21, 2006 .
• moteallehi, mohammad hosein, Sayed-Amir Marashi, and Sedigheh Asad. "Metabolic engineering of E. coli for increasing malonyl-CoA as the limiting metabolite in phenylpropanoid production pathway." 2nd international and 10th national biotechnology congress of Islamic republic of Iran, Tehran, August 29-31, 2017 .
• ghasemi, ali, Sedigheh Asad, Mahboubeh Kabiri Renani00, and Bahareh Dabirmanesh. "Evaluating anti-cancer properties of recombinant L-asparaginase of Halomonas Elongata." 2nd international and 10th national biotechnology congress of Islamic republic of Iran, Tehran, August 29-31, 2017 .
• khodakarami, atefeh, Bahareh Dabirmanesh , Khosro Khajeh, and Sedigheh Asad. "intein mediated gene cloning, expression and purificationof carboxypeptidase G2." 19th national and 17th international congress of biology, Tehran, September 30-1, 2016 .
• Ghasemi, Ali, Sedigheh Asad, Mahboubeh Kabiri Renani00, and Bahareh Dabirmanesh. "A Novel L-asparaginase Candidate for Acute Lymphoblastic Leukemia Treatment (ALL)." Royan International Twin Congress 12th Congress on Stem Cell Biology and Technology, Tehran, September 31-2, 2016 .
• Delara Soltan Esmaeili, and Sedigheh Asad. "Cloning and Cytosolic Expression of Recombinant Urate oxidase in Pichia pink." 3rd International & 11th National Biotechnology Congress of Islamic Republic of Iran, Tehran.
• Najmeh Seyedkatooli, Sedigheh Asad, Sayed Amir Marashi , and Khosro Khajeh. "A metabolic network analysis toward model-driven medium optimization for enhancement of recombinant uricase expression in Escherichia coli." 3rd International & 11th National Biotechnology Congress of Islamic Republic of Iran, Tehran.
• Reyhaneh khaleghi, Sedigheh Asad, and Khadijeh Alishah. "Comparison of the Cytosolic and Secreted Expression of Recombinant Urate oxidase in Pichia pastoris." Annual Conference of the Association for General and Applied Microbiology, Mainz.
• sepideh tamimi, Hamid Moghimi, Sedigheh Asad, Seyed Mohammad Mehdi Dastgheib, and Khadijeh Alishah. "Studies on the Importance of Leader Sequence in the Expression and Secretion of Recombinant Uricase in Pichia pastoris." Annual Conference of the Association for General and Applied Microbiology, Mainz.
• Motahareh Sobat, , Sedigheh Asad, and Khadijeh Alishah. "Identification and extraction of L-asparaginases through large-scale screening of Caspian Sea metagenomics data." Annual Conference of the Association for General and Applied Microbiology, Mainz.
• Khadijeh Alishah, Ralf Holger Voss, Mustafa Diken, Matthias Birtel, Sedigheh Asad, Jamshid Hadjati, and ugur sahin. "CRISPR/Cas9-mediated TβRII disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells in vitro." 18th CIMT annual meeting, Mainz.
• Motahareh Sobat, Sedigheh Asad, , and . "Cloning and expression of L-asparaginase based on Caspian Sea metagenomics data analysis." 3rd International & 11th National Biotechnology Congress of Islamic Republic of Iran, Tehran.